How do CADS convert an analyte to a detectable signal?

All CADs utilize evaporative technology and the conversion of an analyte to a detectable signal involves the same successive steps: 1. Nebulization: Charged Aerosol Detection begins by nebulizing the column eluent into droplets and subsequently drying the droplets into particles. The particle size increases with the amount of analyte. 2.

What is a charged aerosol detector (CAD)?

The Charged Aerosol Detector (CAD) is a detector used in conjunction with high-performance liquid chromatography (HPLC) and ultra high-performance liquid chromatography (UHPLC) to measure the amount of chemicals in a sample by creating charged aerosol particles which are detected using an electrometer.

Why do chromatographers make noise when using a CAD detector?

While most chromatographers are well-versed in identifying and providing a remedy for a noisy baseline with optical detectors, the sources of noise for a CAD typically have a diferent root cause. This results from the fundamentally diferent working principles and selectivity of charged aerosol detection compared to optical detectors.

How does HPLC CAD work?

Detection: charged aerosol particles transfer to a collector, where an extremely sensitive electrometer measures the aggregate charge. This process generates a signal directly proportional to the mass of the analyte present. Discover how the CAD can benefit your HPLC analysis. Learn best lab practices for setting up your HPLC-CAD system.